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For each assay, cells were plated in duplicate, with one plate containing CellTox Green Dye added according to the Express, No-Step Addition at Seeding protocol described in the CellTox Green Cytotoxicity Assay Technical Manual #TM375 (10l of CellTox Green Dye per 5ml of cells)

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It consists of 15 amino acids (a pentadecapeptide) and has been extensively studied in animal models for its tissue-healing and protective properties156

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This movement, called migration, is heavily dependent the protein actin